Abstract: Based on the undergraduate teaching of animal biology experiment and microbiology experiment, the continuity and timing of the two courses in the connection of course content, class arrangement and the use of experimental materials were utilized to organically integrate part of the experimental content of the two courses to form a cross-course comprehensive experimental teaching content. Through this integration, students can be further systematically trained in animal morphology anatomy and classification, aseptic operation, anaerobic bacteria culture and animal intestinal microbial structure cognition on the basis of previous independent experimental operation training. Through the training of experimental operation and the penetration of experimental content, experimental design ability, experimental operation ability, comprehensive application ability of theoretical knowledge and innovation ability were improved, and students were guided to design innovative entrepreneurship projects and organize relative applications, so as to effectively improve the experimental teaching effect and stimulate students’ interest in scientific research.