李佛生, 汪红, 牛壮, 赵天德, 肖渝霖, 吴宛峰, 郑冬超. 小叶榕叶片挥发物抑菌活性的研究[J]. 实验科学与技术, 2020, 18(4): 39-43. DOI: 10.12179/1672-4550.20190263
引用本文: 李佛生, 汪红, 牛壮, 赵天德, 肖渝霖, 吴宛峰, 郑冬超. 小叶榕叶片挥发物抑菌活性的研究[J]. 实验科学与技术, 2020, 18(4): 39-43. DOI: 10.12179/1672-4550.20190263
LI Fosheng, WANG Hong, NIU Zhuang, ZHAO Tiande, XIAO Yulin, WU Wanfeng, ZHENG Dongchao. Study on the Antimicrobial Activity of Volatile Compounds from Ficus microcarpa Leaves[J]. Experiment Science and Technology, 2020, 18(4): 39-43. DOI: 10.12179/1672-4550.20190263
Citation: LI Fosheng, WANG Hong, NIU Zhuang, ZHAO Tiande, XIAO Yulin, WU Wanfeng, ZHENG Dongchao. Study on the Antimicrobial Activity of Volatile Compounds from Ficus microcarpa Leaves[J]. Experiment Science and Technology, 2020, 18(4): 39-43. DOI: 10.12179/1672-4550.20190263

小叶榕叶片挥发物抑菌活性的研究

Study on the Antimicrobial Activity of Volatile Compounds from Ficus microcarpa Leaves

  • 摘要: 为了研究小叶榕叶片挥发物的抑菌活性,该文以小叶榕叶片挥发物吸收液和叶片研磨物为材料,通过平板计数法和涂布密封法检测其对指示菌种金黄色葡萄球菌的抑菌效果;并通过固相微萃取结合气相色谱−质谱联用法检测叶片研磨物中的有效抑菌成分。结果显示:小叶榕叶片挥发物吸收液和叶片研磨物均对金黄色葡萄球菌具有明显的抑菌作用,且抑菌效应与叶片生物量呈正相关关系,新叶的抑菌效果高于老叶;叶片中所含的有效抑菌成分主要是己烯醛和己烯醇类,两者比例的不同可能是造成新叶与老叶抑菌效应差异的原因。本研究证实了小叶榕叶片挥发物的抑菌活性,为小叶榕抑菌效用的开发和推广提供了理论依据。

     

    Abstract: In order to study the antimicrobial activity of volatiles from Ficus microcarpa leaves, the absorption solution of volatiles from Ficus microcarpa leaves and abrasives of leaves were used as materials. The antimicrobial activity of volatiles from Ficus microcarpa leaves against Staphylococcus aureus was tested by plate count method and coating sealing method. The effective antimicrobial components in leaf abrasives were detected by solid phase microextraction combined with gas chromatography-mass spectrometry. The results showed that both volatile absorbent and abrasive of Ficus microcarpa leaves had obvious bacteriostatic effect on Staphylococcus aureus, and the bacteriostatic effect was positively correlated with the biomass of leaves. The bacteriostatic effect of new leaves was higher than that of old leaves. The effective bacteriostatic components in leaves were mainly hexenal and hexenol, and the different proportion of these two organic substances might cause the different bacteriostatic effect between new and old leaves. This study has confirmed the bacteriostatic activity of volatiles from Ficus microcarpa leaves, and provided theoretical basis for the development and promotion of bacteriostatic effect of Ficus microcarpa.

     

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