Abstract: There are few comprehensive and exploratory experiments in Microbiology Experiment course in independent colleges, and students generally lack initiative and participation. To solve this problem, a series of opening and innovative experiments on microbes are designed. The experiments base on the luminosity of photobacteria and its wide application in actual toxicity detection. In the experiments, photobacteria are used as the main microbial species, and the experimental contents are well-designed. The teaching objectives include the cultivation of basic skills in microbiology experiments, innovation ability and the ability to analyze and solve problems. The experimental contents include basic modules, advanced modules, and innovative application modules. The modules are relatively independent and related, which can be flexibly adapted to the practical teaching process. Our results showed that the opening innovative experiments based on photobacteria effectively improve students’ enthusiasm for participation and the initiative of in-depth study after class, and promote their ability and innovation.