Abstract: Botany experiment is a basic discipline in need of strong practical abilities, holding the key to improve students’ practical ability and observation ability. Given the problems of botany experimental teaching in Liaoning Normal University, this paper makes an improvement and optimization of experimental teaching contents and assessment methods based on the practice, and restructures the experimental teaching system. The reform aims to fully mobilize the students’ motivation and initiative in botany learning, and upgrade their practical ability, observation ability and comprehensive quality.