Abstract: By introducing virtual-real combined comprehensive design experimental model into pharmaceutical analysis experiment, this paper determines the content of amlodipine besylate tablets by means of high performance liquid chromatography (HPLC), and conducts the methodology inspection utilizing virtual simulation software. Students are guided to design the experimental method independently, accomplish the experimental content under “virtual-real combined” model, summarize the experimental process, and discuss the experimental results. This paper, through transforming traditional experimental teaching model, is poised to mobilize students’ motivation and initiative, stimulate their desire of science exploration, and hone their creative thinking abilities.