Abstract: Environmental monitoring is a professional basic course for environmental major and an applied discipline with equal emphasis on practice and theory, playing a critical role in the whole environmental learning process. Online automatic monitoring technology of water quality is widely used in different nations and regions for real-time continuous monitoring and remote monitoring of the major rivers, lakes, water conservancy projects and other water areas. However, the experimental teaching of environmental monitoring for undergraduates still focuses on manual on-site sampling and laboratory analysis, resulting in poor cohesion between classroom teaching and the practical monitoring application. A teaching model of “online automatic monitoring system in combination with laboratory analysis” for the environmental monitoring experiment teaching is proposed in the paper. The reform and application of the environmental monitoring experimental course can arouse students’ enthusiasm and initiative, and foster the practical and innovative talents that better meet the needs of society.