HE Qiuxiang. Study on the Method of Separating Stable Isotope DNA Based on Density Gradient Centrifugation Technology[J]. Experiment Science and Technology, 2020, 18(2): 32-35. DOI: 10.12179/1672-4550.20190058
Citation: HE Qiuxiang. Study on the Method of Separating Stable Isotope DNA Based on Density Gradient Centrifugation Technology[J]. Experiment Science and Technology, 2020, 18(2): 32-35. DOI: 10.12179/1672-4550.20190058

Study on the Method of Separating Stable Isotope DNA Based on Density Gradient Centrifugation Technology

  • Unlike the traditional enrichment, separation and purification technology to obtain some purely cultured pollutant-degrading functional microorganisms, stable isotope technology (SIP-DNA) can more easily identify functional genes. In this study, the core technology of density gradient centrifugation in SIP-DNA was used to obtain the conditions and related parameters of the technology. The buoyancy density and DNA concentration results show that this method can effectively isolate isotope-labeled and non-isotopic-labeled DNA and lay the foundation for subsequent high-throughput sequencing of SIP-DNA. It also provides technical means for centrifuge managers and experimenters to use the method correctly.
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