Laser Scanning Confocal Microscopy and Its Application

Laser scanning confocal microscopy has become crucial to biological and medical research. To fully and flexibly use the function, improve the application and performance, and boost the potential demand of confocal microscope, this article introduces the fundamental principle, instrument configuration and technical parameters, sample preparation requirements, image acquisition procedures and techniques, as well as daily maintenance and management information of Nikon A1⁺ laser scanning confocal microscope. The aim is to provide a powerful technical reference for scientific researchers to obtain high-quality confocal images, improve work efficiency, and better assist teaching and scientific research.