Technical Study on Silkworm Clearing Using Methyl Salicylate

The tissue display of silkworm using tissue clearing technique is of special significance to sericology teaching and relevant scientific research. This paper introduces a technique on silkworm clearing using methyl salicylate. The results have shown that the effect of methyl salicylate on silkworm clearing is good; the four kinds of dyeing could improve the contrast of silkworm tissues, and overall effect follows the order of methylene blue>hematoxylin>toluylene red>eosin. The dyeing of toluylene red and eosin on silkworm tissues are uneven, providing little contrast; the dyeing of hematoxylin is obvious with even coloring, clear display, yet unobvious overall contrast. The dyeing of methylene blue on different tissues of silkworm is heterogeneous and easy to detect.