Imaging Techniques and Application of Super-resolution Laser Scanning Confocal Microscope

Super-resolution laser scanning confocal microscope has been widely used in the field of life science research. Taking Zeiss LSM880 Airyscan with STEDYCON super resolution laser scanning confocal microscopy(LCSM) as an example, this paper introduces the principle, application and parameter setting of two super resolution technologies Airyscan and stimulated emission depletion (STED) based on stimulated emission phenomenon, in order to provide a reference for the scientific research workers on which mode to adopt for image acquisition to obtain the best imaging effect.