Design and Practice of the Comprehensive Experiment “Induction and Detection of Autophagic Flux”

One of the important ways to improve the experimental teaching level and the talent training quality is dismantling, refining and adding the international frontier theories and technologies to the experimental teaching in time. The comprehensive experiment of induction and detection of autophagic flux was designed, which included cell passage culture, cell transfection and induction and observation of autophagy. A systematic, comprehensive and exploratory modular experimental teaching content was formed. Through Double fluorescent labeled autophagosome marker protein expressed in cells and starvation inducing, autophagosomes and autophagic flux were observed by fluorescence microscope. The practice results in undergraduate experimental teaching indicated that it was helpful to cultivate students' scientific research thinking, cultivate students' innovative spirit , and improve students' ability to analyze and solve problems.