Researching Experiment Design of Chromatography Technology in Animal Nutrition Experimental Course

Chromatography is an important part of the research experiments by students in the major of Animal Science when they study the course Animal Nutrition Experiment. Under the guidance of the teachers, the student team independently designed the experimental scheme by combining the scientific research project and the actual production, and successfully established a method for the determination of GMP and IMP in feed flavoring agent using high-performance liquid chromatography. After the sample was pre-treated, the isocratic elution was carried out on an Athena C₁₈ column with methanol and 0.05 mol/L potassium dihydrogen phosphate solution (2:98, v/v) as the mobile phases, and the flow rate was set at 0.6 mL/min. The temperature of the chromatographic column was controlled at 25 ℃, the detection wavelength was set at 250 nm, and the injection volume was 10 μL. The experimental results showed that the concentrations of GMP and IMP showed a good linear relationship with the peak areas in the range of 0.05 mg/mL to 2.0 mg/mL (R²=0.9999), with the detection limits of IMP being 0.4 μg/mL, and that of GMP being 0.8 μg/mL. In addition, the recoveries of the method were as high as 98% with the relative standard deviations of 0.81% and 0.75%, respectively. The experimental program is not only easy to operate and the analysis time is reasonable, but also has high precision and high recovery, which can help to stimulate the students’ interest in learning and the spirit of exploration, as well as cultivate their innovative ability and scientific literacy.