Comprehensive Experimental Design for Glucose Detection by Co/NC Nanozymes

In this paper, a comprehensive experiment is designed to synthesize Co/NC nanozymes derived from metal-organic frameworks for the detection of glucose content. A series of instruments are used to characterize Co/NC nanozymes. The results show that when the ratio of Co to Zn was 1∶1, the carbonation temperature is 900 ℃, and pH is 3.5, the Co/NC nanozymes have the greatest oxidase-like activity. The maximum reaction rate of Co/NC nanozymes is 1.74×10⁻⁷ M/s, and the nanozymes have a strong affinity for substrate TMB. The linear range of glucose detection is 1-60 μM, and the monitoring system have excellent selectivity. The experiment contains multidisciplinary knowledge points, which is helpful for students to understand the knowledge system, open up students' thinking, improve their innovation ability, and cultivate their scientific literacy.