Research of Different Extraction Process of Polysaccharide From TaiBai Fritillariae with Orthogonal Test

To study the extraction process of polysaccharide in TaiBai Fritillariae(PFT),the polysaccharide was extracted by water bath,ultrasonic and boiling method,and the content was assayed by enthrone colorimetric. Using orthogonal experiment analysis,the optimal extraction conditions of three extraction process were as follows: the water bath extraction method: temperature 70 ℃,solid-liquid ratio 1∶15,and extraction time 1 h,content of the optimum conditions of extraction of polysaccharide was 0.65 mg/g; The ultrasonic cell crushing extraction method: temperature 80 ℃,solid-liquid ratio 1∶25,and extraction time 15 min,content of the optimum conditions of extraction of polysaccharide was 0.99 mg/g; The boiling method: temperature 90 ℃,solid-liquid ratio 1∶20,and extraction time 3 h,content of the optimum conditions of extraction of polysaccharide was 0.38 mg/g.