Abstract: Combined with research achievements of organic light-emitting device (OLED), this paper explored a research-based comprehensive chemistry experiment which included the preparation and characterization of OLED with different structures. The comprehensive experiment is based on the structures, preparations and characterization of OLED to cover the materials selection, substrates cleaning, vacuum evaporation, masking skill, photoelectric characterizations and so on. The content of experimental teaching is the interpenetration and intersection of physics, materials and chemistry, and it involves knowledge in the fields of organic electronics, materials physics, semiconductor devices and optoelectronic technology. Through this experiment, students can improve their experimental ability, cultivate scientific thinking, creative ability, and solve practical problems, so as to better play the role of experimental teaching and cultivate innovative talents and industrial technical talents.