Abstract: Animal germ cell (oocyte, embryo, sperm) cryopreservation technology has played an important role in animal husbandry, clinical medicine and biotechnology. Using ultra-low temperature cryopreservation methods to preserve germ cells of rare and endangered transgenic animals can achieve long-term preservation of animal germplasm resources. However, the cryopreservation of germ cells and tissues of disease animals still have many problems. This article describes the practical problems and selection of germ cells and tissues of animal strains to be preserved in cryopreservation, in order to promote the application of cryopreservation technology in animal resource preservation.