Abstract: “Environmental monitoring experiment” is a core curriculum for the undergraduate students in the major of environmental science or environmental engineering. In order to better train the students to be the integrated talents with both excellent practice skills and outstanding innovative capacity, comprehensive experiment projects aiming at enhancing the students’ innovative capacity are of great necessity. The present study explores the teaching design of one of the comprehensive experiments of “determination of mercury content in hairs”. The teaching strategy and design are continuously improved. Under the guidance of outcome-based education (OBE), whole-process evaluation including the experiment design and preparation, operation and observation, data analysis and discussion is established for the score assessment.