Abstract: Microfluidic chips used as experimental platforms have promoted the miniaturization of complex biochemical experiments, with advantages such as high integration, high sensitivity, high controllability of experiments, easy observation and analysis, and portability. In order to achieve precise control of micro-flow, a micro-flow pump control system based on STM32 was developed. This system can achieve a flow control accuracy of 10 μL/min. The system uses STM32 as the main control chip, controls the piezoelectric pump driving chip DRV2667 and the differential pressure sensor SDP31 through the I²C bus, and displays the flow value on the OLED display screen. The entire device has a volume of 17.4 cm×13.4 cm×2.7 cm, making it small and easy to carry. Measurement results have proven that this system can be applied to microfluidic experimental devices.