Abstract: To fully leverage the role of scanning electron microscopy (SEM) in the cultivation of innovative talents, a virtual simulation system has been introduced into the open experimental course of Micro World under Scanning Electron Microscopy, which solves the problems existing in the traditional open experimental teaching. The powerful functionality and interest of the virtual simulation system can effectively mobilize student’s initiative and enthusiasm for learning. By navigating through the increasingly challenging teaching-learning process of “independent learning−mastering knowledge−designing practical content−completing experimental inquiry”, students will actively construct relevant knowledge systems, stimulate innovation awareness, and improve scientific research innovation abilities. In this way, a multi-level experimental teaching mode of “irtualization first, reality second, supplementing reality with virtualization, and combination of virtualization and reality” is thus formed, which broadens and deepens the scope of open experimental teaching, showing excellent teaching practice results.