Abstract: The interactive video virtual program is a computer application that utilizes video as its core medium and skillfully integrates various media forms such as images and text. Depending on the user’s different operations, the program can provide varied feedback. It boasts several key advantages, such as a short production process, low equipment requirements, and vivid content presentation, playing a positive role in experimental teaching. This study, based on the molecular identification experiment of traditional Chinese medicine, developed a program using the Unity platform and applied it to the teaching practice of the molecular pharmacognosy experimental course at the School of Pharmacy, Tongji Medical College. The findings demonstrate that this interactive video virtual program effectively enhances teaching quality and yields satisfactory educational outcomes.