Abstract: Based on the concept of green chemistry, the traditional experiment for determining ultrafiltration membrane separation capability was improved from multiple aspects, including the selection of raw materials and chromogenic agents, the experimental scale, the dosage of reagents, and the waste liquid treatment. Through a series of effective measures, such as the use of green raw materials and reagents, microscaling of the experiment, and waste liquid recovery, the economic efficiency of the experiment was significantly enhanced, achieving a fully green process. The implementation of the improved ultrafiltration membrane separation experiment not only helps enhance students’ professional skills and raise environmental awareness while fostering the concept of green chemistry, but also serves as a typical case for ideological and political education in green chemistry.