Abstract: To address the problems in experimental teaching of Petroleum and Natural Gas Engineering under new business formats, such as fragmented projects, insufficient challenge, and disconnection from industrial frontiers, this paper conducts reform research aligned with industry development needs. An independent experimental course system featuring “systematized knowledge, specialized content, and cutting-edge information” is established, with optimized project types and class hours, and the integration of virtual simulation experiments to enhance teaching levels. Teaching methods such as “internal cultivation and external expansion,” “Lego-style modularization,” and “progressive question guidance” are applied. Meanwhile, experimental teaching aids are improved, a teaching feedback mechanism is established, and students’ innovation and inquiry abilities are strengthened. The assessment method is optimized to emphasize process and design evaluation, the incentive system is improved, and students’ autonomous learning ability is cultivated. This model effectively improves the quality of experimental teaching and students’ innovative research capabilities, providing new ideas for experimental teaching reform.