Abstract: Conventional laboratories are burdened with the scarcity of digital resources and inefficiency of management in terms of education, teaching and safety management. Promoting informatization construction for university laboratories is an efficient way to enhance their comprehensive service capabilities and the students’ innovative and practical abilities. In response to the current situation of the Zhejiang Provincial Experimental Teaching Demonstration Center, which suffers from insufficient digital teaching resources, low degree of intelligence in its spatial environment, and outdated safety control measures, this paper explores the guiding significance of a construction plan based on the EHS concept and supported by information technology. It also elaborates on the exploratory practice of laboratory informatization construction in terms of strengthening virtual and physical teaching resources, building an intelligent environmental system, and introducing a digital platform for laboratory safety management. The research and practice have shown that through the coordinated operation of various information systems, laboratories can be operated safely and stably. As a result, the quality of teaching and services can be improved and the need to cultivate innovative engineering talents can be effectively addressed.