Abstract: Neuroscience typically relies on experimental animals as research subjects, and their quality directly affects the scientific validity of research results. This paper examines the characteristics of animal housing space construction technology in domestic neuroscience laboratories, taking into account the welfare and quality requirements of experimental animals, and analyzes the construction requirements for animal housing in neuroscience laboratories. By examining specific engineering examples, the corresponding construction techniques are elaborated in detail. This work can provide references for the construction of animal housing spaces in similar neuroscience laboratories and promote the development of related disciplines.