Abstract: In response to the urgent demand of enterprises for high-end engineering and technical talents in intelligent manufacturing, digital twin, production line optimization, etc. under the background of intelligent factory construction, and in order to solve the problems of poor systematization, high practical costs, and single majors of students in current engineering training and practical teaching, an intelligent factory practical training platform combining virtuality and reality has been designed and constructed, a practical teaching system for intelligent factory production line optimization has been developed, and multi-dimensional practical assessments have been carried out. By integrating methods such as virtuality and reality combination, product lifecycle management, value stream mapping, and process data analysis, it enhances students' systematic thinking and their ability to solve complex engineering problems. The practical results show that the practical mode combining virtuality and reality has effectively improved the teaching quality of intelligent factory practice. This practical teaching system can more efficiently achieve the optimization goals of intelligent factory production lines and provide a new idea and path for the practical teaching of intelligent manufacturing.