Abstract: Laboratory animals, as living research materials with clear genetic background and microbial quality control, are indispensable strategic support in the fields of life science research and medical achievement transformation. The Laboratory Animal Center has become a critical position for universities to train top-notch innovative talents, build first-class discipline ecology and improve the level of scientific research. Under the background of “interdisciplinary integration”, university laboratory animal centers are facing the challenge of transforming from traditional service mode to integrated and intelligent. Taking the construction of Laboratory Animal Center of Sichuan University as an example, this article explores the key issues in the construction of Laboratory Animal Center from the aspects of reform of Laboratory Animal Science curriculum, new model of laboratory technology service, animal welfare and ethics, and management costing, which provide a new ideal for the construction and development of “double first-class”.