Abstract: The high-resolution transmission electron microscope (HRTEM) serves as a pivotal tool for materials characterization, and its sharing efficiency directly impacts the allocation of research resources and innovation performance. This study, based on the JEOL JEM-2100F high-resolution transmission electron microscope, establishes and implements a four-dimensional sharing system comprising “professionalized management, standardized training, information-based services, and an efficient technical team.” This system shifts the equipment from passive accessibility toward proactive scientific support. After implementation (with 2021 as the baseline), by 2024, the effective operating time of the instrument increased by 80.2%, the number of served users grew by 97.1%, supported research projects rose by 33.3%, and paper output backed by the instrument expanded by 150%, significantly enhancing both equipment utilization and research support capacity. Characterized by “core stability and dimensional flexibility,” the system offers a transferable management framework for the shared use of similar large-scale instruments. It provides a valuable reference for promoting intensive allocation of scientific resources and improving overall innovation effectiveness.