Abstract: The effect of rotation angel on different concentration sucrose solution in different light sources is measured. The modified instrument can analyze the trend of rotation rate changes with the light source wavelength. Through rotating polarization analyzer, the sucrose solution attribute of left rotation or light rotation is measured. It is proven that the experiment instrument is used for a more intuitive and more profound understanding of the effect rotation, which enriches and expands the experiment teaching contents. In addition to the liquid media, the measurement method is also suitable for the solid media.