Abstract: Experiment teaching exists some problems, such as experiment was attached to theory, experiment hours were less, experiment content was most of the verification experiment, teaching model was single, merit system was not perfect, etc. Thus, we put forward the reform plans of setting course on environmental monitoring experiment independently, increasing the experiment content of comprehensive, design and research, improving the experiment teaching model,reforming the experiment merit system and improving the experiment teaching conditions. The practice showed that the new system which was reformed on environmental monitoring experiment teaching could improve the quality of experiment teaching, and cultivate students’ ability of practice, analyzing and solving problems, innovation and scientific research.