Abstract: Some useful explorations and practices in green organic chemistry experiment have been carried out to improve the teaching quality. These measures include reasonable arrangement of multi-step synthesis experiments, using green catalysts, designing of the waste recycle, and micro-experiment. The optimization of contents and establishment of the course selection platform for open experiment have provided supports for greenization teaching. As a result, practices of green teaching in organic chemistry experiment enhance the students’environmental awareness, comprehensive quality and creative ability.