Abstract: In order to better understand intuitively the process of reconstructing B-mode images from focused ultrasound RF signal for undergraduate students, an experiment is well designed.Firstly, it makes students complete the collection of RF data on ultrasound equipment.Secondly, it completes imaging process simulation by analyzing the collected signals in each imaging module on MATLAB platform.Finally, it compares the obtained results with shown image in ultrasound equipment.This experiment allows students participating in designing with an opening result.The practical result shows that:the experiment process can not only help students increase the ability to analyze certain problem, but also deepen students understanding of the curriculum, stimulate their interest in learning.Thus this experiment will achieve the purpose of improving the teaching effect and quality.