Abstract: With the growing awareness of environmental protection, the establishment of green biological laboratory has become an important part in laboratory management and reform.According to the realities of the biological laboratory in the universities, guided by the theory of green environmental protection, the ways and methods for“green biology laboratory” were explored from the green biological laboratory ideas, constructing the green environmental teaching,attaching importance to the collection and treatment of the wastes, strengthening laboratory management.These work achieve good effect.