Abstract: Comprehensive experimental teaching plays an important role in the students practical ability,innovation ability and comprehensive quality training.Based on the scientific research projects,this paper discussed the design,practice and effect of the comprehensive experimental project in plant ecophysiology.As a result,the reform practice effectively improved the learning initiative and enthusiasm of students,trained the ability of scientific research,and independently analysis and problem solving ability,achieved a good teaching effect.As a new mode of experiment teaching,it is a useful way for the experiment teaching.