Abstract: Combined the scientific research of teacher with chemical experimental teaching, a research type chemistry teaching experiment-the detection of As3+ in river water by resonance light scattering based on gold nanoparticles, was explored and designed.This item was transformed from research subjects.This experiment could satisfy the requirements of the stability and reproducibility in teaching experiment, meanwhile, there are many opening research topics including different preparation methods of gold nanoparticles, the correlativity between the size and optical characteristic of gold nanoparticles, and other target analytes dependence on modification molecular encapsulated on gold nanoparticles, therefore, this experiment could be used in opening chemistry experimental teaching.Through this proposed experiment, the students could understand and grasp the working principle and operation skill of UV-vis spectrometer and spectrofluorimeter, meanwhile, they also could understand the preparation methods and fluorescence characterization techniques of gold nanoparticles, and develop their comprehensive ability, relevant scientific literacy and research ability.