Abstract: In view of the problems existed in the traditional experimental teaching of assembly language course of mainframe,this paper makes some exploration on the experiment contents,experiment process and experiment evaluation.Following the teaching mode of overturning the classroom,a new experimental teaching model,which takes the students as the center,is constructed according to the teaching mode of flipped classroom,and specific experimental teaching examples and implementation processes are given during the teaching practic.The purpose of the reform is to enable students to master students' hands-on and innovative abilities through practical programming in addition to mastering mainframe hardware knowledge and compiling instructions.