Abstract: To train the innovative talents with abilities of independent-learning, innovation consciousness and ability, this manuscript constructs an experiment teaching mode that combines MOOCs and chemical laboratory. Furthermore, we take “the design of fluorescent sensor of iron ion and application” as the specific teaching case to propose the specific implementation measures of this mode. The experiment teaching mode adopts the inquiry learning on line (research background, rudimentary knowledge, operating skills), and practices operation in laboratory. Then, the students design research program and carry out the scientific researches under the guidance of teachers. This reform mode emphasizes that students find and solve problems in inquiry learning by themselves, so as to develop their independence (self-educated ability and self-design technique), motivation and creativity with the guidance of teachers. And it applies to advanced undergraduates and postgraduates.