Abstract: With the development of genetic engineering and maturity of related technologies,genetic engineering has become a very practical and very popular mandatory course during the undergraduate period.However,it is difficult for students to master experimental the skills in a limited class time because of its long experimental period,complex contents and comprehensiveness.Improving teaching skills can be a useful exploration to solve this problem.Interactive Flash animation was used in this experiment teaching,trying to effectively integrate text,graphics,images and other related content through animation and game to improve students' ability to understand abstract experimental principles and master cumbersome experimental steps.Through questionnaires,interviews and practical application,it is proved that interactive Flash has achieved the expected teaching effect in genetic engineering experiment teaching.