Abstract: In this paper, a comprehensive experiment is designed to synthesize Co/NC nanozymes derived from metal-organic frameworks for the detection of glucose content. A series of instruments are used to characterize the Co/NC nanozymes. Through control variable method, kinetic tests, and standard curve method, the testing conditions of nanozymes and the detection range for glucose are optimized. The experimental results show that when the molar ratio of Co to Zn is 1:1, the carbonization temperature is 900 ℃, and pH is 3.5, the Co/NC nanozymes exhibit the greatest oxidase-like activity. The maximum reaction rate of Co/NC nanozymes is 1.74×10⁻⁷ mol·L⁻¹·s⁻¹, and the nanozymes demonstrate a strong affinity for the substrate TMB. The linear range for glucose detection is 1~60 μmol, and the detection system exhibits excellent selectivity. The experiment integrates multidisciplinary knowledge, helping students achieve a comprehensive understanding of the knowledge system, develop scientific research thinking, improve innovation ability, and cultivate scientific literacy.