Abstract: University laboratories are important scientific research places in universities, where researchers frequently launch research work. The chemical laboratory involves a wide variety of chemicals, dangerous equipment and different chemical reactions. Based on these characteristics and current situations, 5S management (seiri, seiton, seiso, seiketsu, shitsuke), a method originated from Japanese companies, is playing a role in routine management of the university chemical laboratories to improve the environment of laboratories and promote the quality of experimenters. The application of 5S management effectively improves the utilization rate of laboratory space and work time, optimizes research environment, reduces the potential safety hazards, and improves the management level of laboratories.