Abstract: The following problems exist in the course of electronic design automation courses, for example, the use of software platform is boring, lack of comprehensive system teaching project cases, electronically aided design (EDA) and virtual simulation and hardware implementation have some disconnected issues. Taking the design of M-sequence signal generator as a comprehensive case, this paper analyzes and deduces the characteristics of multi-order m-sequence signals, and forms a sequence signal generator circuit through 555 timer, double-D flip-flop, logic gate circuit and operational amplifier circuit. Taking the 6th order m sequence as an example, the output waveform of each link is determined by Multisim software simulation, and the PCB is designed assisted by the software Alttitum Designer. The experimental test results are completely consistent with the simulation, which confirms the correctness of the design and gives relative explanation aiming at problems during debugging. Through comprehensive teaching experiments, students can better master the use of EDA software and hardware assembly, debugging and testing, and improve the comprehensive skills of electronic technology.