Abstract: Transistor amplifier circuit is the most important and difficult point in the comprehensive experimental course of electronic circuits. It has strong practical significance and plays an important role in improving students’ electronic system design and practice. This article proposes a topic of using transistors to make operational amplifiers, which basically includes typical transistor amplifier circuits in analog electronics, such as constant current source differential amplifier circuits, constant current source circuits, common-emitter amplifier circuits, and complementary output circuits. Then the system scheme and circuit parameters are designed through theoretical calculation and Multisim simulation. Finally, various technical indicators were measured and analyzed. The experimental results show that the design scheme is clear and shows the principle and working process of the transistor amplifier circuit, and the teaching effect is good, which can provide some reference and help for other universities to set up related courses.