Abstract: The sandbox simulation of business operation is a comprehensive business management experiment for students majoring in business administration. In the implementation of experimental teaching, there are problems such as the professional role of the production director not being effectively experienced, production planning and production scheduling management not being valued, and inventory decision-making knowledge not being practically internalized. The reconfigurable manufacturing execution system (RMES), a workshop management information system, can be used to realize the contextualization of experimental teaching and high simulation of enterprise operation just like the sandbox. In order to strengthen the students’ learning of enterprise production management in the simulation of business operation sandbox, three preliminary experimental items of assembly production process analysis, production cycle setting, assembly line balance, and station feeding rules were designed to enrich the experimental teaching content by using the RMES platform.