Abstract: Aiming at various problems in genetic engineering experimental learning, an attempt was made to fuse the elements of “gamification” with virtual technology to develop genetic engineering experimental teaching software “genetic engineering game experiment (GEGE)”. Compared with the virtual experimental platform, GEGE software focuses on the educational function of training students to mobilize higher-order thinking to solve problems in a game-breaking way. The teaching practice results show that the GEGE software has played a positive role in helping learners to establish a connection between macro operations and micro results, promoting imagination, and enhancing the use of knowledge. This attempt provides ideas for promoting digital game teaching in higher education biology majors.