Abstract: Given the current problems in cotton fabric dyeing such as large water consumption, energy consumption, high chroma of wastewater and large pollution, this paper makes transformation and exploration on experimental teaching content. Multi-amine compounds and detergent-Y are applied to dye cotton fabric with reactive dyes and clean floating colors. The results show that the color depth and color fastness are close to or better than that of soaping fabric samples, especially the fastness to hot water; the absorbance of hot water residue is zero, meanwhile the soaping temperature drops from 95℃ to 70℃, and the dye strength of dyeing wastewater is at a low level. This paper uses the experimental results to make transformation and exploration on current experimental teaching, and carries out feasibility and safety analyses.