Abstract: In order to select a safe, economical and effective buffer solution and medium for counter-immunoelectrophoresis (CIEP), six buffer solutions with pH value of 8.6 were selected from common buffer solutions for electrophoresis, with agar and agarose used respectively as the electrophoresis media, to observe the formation of white precipitation line of antigens and antibodies after electrophoresis. The results showed that Tris-HCl buffer was the best of the six buffer solutions. Both agar and agarose gels produced significant white precipitation lines, and the white precipitators became shallower with the decrease of antigen concentration. The effects of agarose used as the electrophoresis media in barbiturate and barbiturate sodium, TAE, Gly-NaOH and NaCl-NaOH buffers were better than those of agar. The TGS buffer was the worst. It was concluded that Tris-HCl, Barbitone and barbitone sodium, TAE, Gly-NaOH and NaCl-NaOH buffer could all be used as buffer solution for counter immunoelectrophoresis. However, Tris-HCl was the best, followed by TAE and Gly-NaOH, in terms of safety, economy and experimental effect. Therefore, Tris-HCl will be used as buffer solution for counter-immunoelectrophoresis in the future immunological experiments.