Abstract: In order to meet the needs of the development of electronic information industry for innovative talents, this paper follows the hierarchical collaborative concept of “strengthening basic experiment teaching, focusing on the guidance of engineering project, and promoting engineering quality improvement”, and constructs the practical innovation ecosystem of electronic information college students. The core elements include the interaction between the main entities of cultivating innovative talents, the free mobility of innovative practice talents cultivation resources and the activity carrier construction of students’ innovation ability cultivation. The promotion and application effect of the practical innovation ecosystem shows that the ecosystem can effectively promote the communication, interaction and cooperation between teachers and students, between students, and achieve a high degree of adaptation, coordination and progress. Finally, the self-management, self-service and self-improvement of college students based on the science and innovation platform can be realized, the practical innovation ability of college students can be cultivated, and the talent training level of local universities can be improved.