Abstract: The cultivation of innovative talents is an important goal of the undergraduate education, and the experimental teaching is a key link in the creative talent cultivation. In view of the problems and deficiencies in the experimental teaching, the teaching reform of optical fiber sensing experiment is conducted. In the experiment process, we should take students as the main body and give teachers full play to the guiding role. Teaching concepts, teaching methods, teaching contents and evaluation systems are reformed to arouse students’ enthusiasm. Comprehensive, design and research experimental projects are set up to explore the teaching reform of the optical fiber sensing experiment, improve the students’ initiative in learning and teamwork ability, and cultivate their innovative consciousness, independent scientific research ability and rigorous scientific research attitude.