Abstract: This paper takes the classic organic synthesis experiment acetylsalicylic acid preparation as an example, introduces the synthetic methodology into the undergraduate organic chemistry experiment teaching. The influences of experimental results are carried out by changing the reaction temperature, heating method, catalyst type and purification method. The comparative research and systematic exploration based on the same experiment can not only allow students to realize the method of scientific research synthetic experiment, but also effectively mobilize their enthusiasm and cultivate their innovation ability, inquiry ability and scientific research ability. At the same time, the use of environmental-friendly green catalysts helps build students’ concept of green environmental protection, which is in line with the concept of green chemistry.