Abstract: This paper draws on Hazard Analysis and Critical Control Points (HACCP), an internationally accredited food safety guarantee system, analyzes all hazards in the whole process of biological experiment from the start and determines the key links of the experiment for identifying potential safety hazards. The HACCP plan is made, and a biological laboratory security control system is established. Having combined with network information technology, the corresponding control and corrective measures are taken for each critical control point during the experiment. The practice has proved that HACCP system provides effective guarantee for the safety of biological laboratory, and also provides new management methods and effective means for the safety management of biological laboratory in other universities.