Abstract: The analysis of single transistor amplifier circuit is an important part of low-frequency electronic circuit course. In the corresponding verification experiment, the analysis of signal distortion mainly adopts the method of oscilloscope observation and adjustment of potentiometer and signal source. The analysis and explanation of distortion phenomenon is not carried out in a detailed and comprehensive manner, therefore the measurement error of maximum undistorted output is large. Through the deep exploitation of Multisim software, combined with the simulation results of specific circuit cases, the signal distortions are described quantitatively and the experimental results are fully verified and extended. At the same time, combined with the comprehensive simulation function of Multisim, the auxiliary design method of the software is proposed for the measurement of the maximum undistorted output, so as to reduce the measurement error. The results show that simulation design ideas and results are instructive to the optimization of the experimental method.